BRAF Antibody BRAF/1626 / B-Raf Kinase Antibody [clone BRAF/1626] (V4594)

BRAF Antibody BRAF/1626 Detection Across Human Tissues and Cancer Cell Lines WB. Western blot analysis of human brain, intestine, kidney, lung, liver, pancreas, skeletal muscle, skin and stomach tissue lysates, and A375, A431, A549, COLO38, EL4, HCT116, HEK293, HeLa, HepG2, Jurkat, K-562, KATO III, MCF-7, MDA-MB-231, PANC28, PC-3, Raji, Ramos, RH30, THP-1, U87 and Y79 cell lysates using BRAF Antibody clone BRAF/1626. A prominent band is detected at approximately 80-85 kDa across the tested samples, consistent with the expected molecular weight of B-Raf kinase.

BRAF Antibody BRAF/1626 Staining of Human Ovarian Cancer IHC. Immunohistochemistry staining of FFPE human ovarian cancer tissue using BRAF Antibody clone BRAF/1626. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before staining. The inset shows the secondary antibody negative control, with PBS substituted for the primary antibody.

Purified BRAF Antibody BRAF/1626 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free BRAF Antibody clone BRAF/1626 under reduced (R) and non-reduced (NR) conditions with 2 ug loaded per lane. Under reducing conditions, bands corresponding to the immunoglobulin heavy and light chains are visible at approximately 50 kDa and 25 kDa, respectively. The non-reduced antibody migrates predominantly near 150 kDa, consistent with intact IgG and supporting the integrity and purity of the purified monoclonal antibody preparation.