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Home >> Antibodies >> BRAF Antibody (B Raf)

BRAF Antibody (B Raf) (R31582)

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  Catalog No Formulation Size Price (USD)  
Image R31582 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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IHC staining of FFPE human placental tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adenocarcinoma of the rectum with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with BRAF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) rat brain, 3) rat testis and 4) mouse brain lysate with BRAF antibody. Expected molecular weight: 85-95 kDa.
Flow cytometry testing of human U-2 OS cells with BRAF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRAF antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P15056
Gene ID 673
Localization Cytoplasm, nucleus, plasma membrane
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This BRAF antibody is available for research use only.
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Description

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

For a monoclonal reagent targeting BRAF, see our BRAF Antibody BRAF/1626 / B-Raf Kinase Antibody page.

Application Notes

Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 38-230) was used as the immunogen for this BRAF antibody.

Storage

The lyophilized BRAF antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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