BOB.1 Antibody [clone BOB1/2422] (V8023)

IHC testing of FFPE human tonsil with BOB.1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human spleen with BOB.1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human Raji cell lysate with BOB.1 antibody. Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using BOB.1 antibody (clone BOB1/2422). These results demonstrate the foremost specificity of the BOB1/2422 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free BOB.1 antibody as confirmation of integrity and purity.