BMI1 Antibody (R31554)

BMI1 Antibody U2OS Cell IF. Immunofluorescent staining of fixed and permeabilized U2OS cells with BMI1 Antibody (red) and an Alpha Tubulin antibody (green). Cells underwent enzyme antigen retrieval for 15 minutes, were blocked with 10% goat serum, and incubated overnight at 4 degrees C with rabbit anti-BMI1 antibody at 5 ug/ml and mouse anti-Alpha Tubulin antibody. Cy3- and Fluoro488-conjugated secondary antibodies were used at a 1:500 dilution. BMI1 exhibits predominantly nuclear staining, consistent with its function as a Polycomb Repressive Complex 1 component involved in epigenetic gene silencing. This BMI1 Antibody is suitable for studies of chromatin regulation, stem cell maintenance, and cancer biology.

BMI1 Antibody Human, Rat and Mouse WB. Western blot analysis of human 293T (lane 1) and K562 (lane 2) whole cell lysates, rat thymus (lane 3), and mouse thymus (lane 4) using BMI1 Antibody at 0.5 ug/ml. A specific band is detected at approximately 40 kDa in all samples, consistent with the expected migration of BMI1, which has a predicted molecular weight of approximately 37 kDa. A characteristic BMI1 doublet is also observed, consistent with the phosphorylation-dependent mobility shifts reported for BMI1 in the literature. This BMI1 Antibody is suitable for studies of Polycomb Repressive Complex 1 function, epigenetic gene regulation, stem cell maintenance, and cancer biology.