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Home >> Antibodies >> BMI1 Antibody

BMI1 Antibody (F53353)

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  Catalog No Formulation Size Price (USD)  
Image F53353-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F53353-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of BMI1 antibody at 1:2000 dilution. Lane 1: SW480 lysate; 2: HeLa lysate; Predicted molecular weight: 37-43 kDa.
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Western blot testing of BMI1 antibody at 1:2000 dilution. Lane 1: SW480 lysate; 2: HeLa lysate; Predicted molecular weight: 37-43 kDa.
Western blot testing of BMI1 antibody at 1:2000 dilution + A549 lysate
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P35226
Applications Western blot : 1:1000-2000
Limitations This BMI1 antibody is available for research use only.
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Description

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Application Notes

Titration of the BMI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This BMI1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 230-265 amino acids from the C-terminal region of human BMI1.

Storage

Aliquot the BMI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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