BMI1 Antibody / Polycomb Stem Cell Renewal Marker [clone BMI1/2823] (V8080)

BMI1 Antibody NIH3T3 WB. Western blot analysis of mouse NIH3T3 cell lysate using BMI1 Antibody / Polycomb Stem Cell Renewal Marker. A distinct band is detected near 37-43 kDa, consistent with the predicted molecular weight range of BMI1. The observed expression pattern supports the chromatin-associated role of BMI1 in polycomb-mediated transcriptional repression, stem cell self-renewal signaling, and epigenetic regulation pathways involved in proliferative cell maintenance.

BMI1 Antibody Colon Carcinoma IHC. Immunohistochemistry analysis of FFPE human colon carcinoma tissue using BMI1 antibody clone BMI1/2823. Tumor epithelial cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated localization of BMI1 in polycomb-mediated transcriptional repression, stem cell self-renewal signaling, and epigenetic regulatory pathways active in proliferative colorectal carcinoma tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

BMI1 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using BMI1 antibody clone BMI1/2823. Tumor cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated role of BMI1 in polycomb complex-mediated transcriptional repression, stemness-associated self-renewal signaling, and epigenetic regulation pathways within proliferative breast carcinoma tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

BMI1 Antibody Prostate Carcinoma IHC. Immunohistochemistry analysis of FFPE human prostate carcinoma tissue using BMI1 antibody clone BMI1/2823. Tumor epithelial cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated localization of BMI1 in polycomb-mediated transcriptional repression, stem cell renewal signaling, and epigenetic regulatory pathways associated with proliferative prostate carcinoma tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

BMI1 Antibody HeLa IF. Immunofluorescence analysis of PFA-fixed human HeLa cells using BMI1 antibody clone BMI1/2823. BMI1 staining (green) demonstrates prominent nuclear localization consistent with the chromatin-associated role of BMI1 in polycomb-mediated transcriptional repression, stem cell self-renewal signaling, and epigenetic regulation pathways. Phalloidin staining (red) highlights the actin cytoskeletal network and cellular morphology.

BMI1 Antibody Protein Microarray Validation. Analysis of a HuProt protein microarray containing more than 19,000 full-length human proteins using BMI1 antibody clone BMI1/2823. The antibody demonstrates highly selective recognition of BMI1 with a Z-score of 91.89 and an S-score of 87.2, supporting exceptionally strong target specificity for this polycomb-associated stem cell renewal regulator relative to other proteins represented on the array. R and CYSLTR1 were identified as substantially lower-ranking signals. Z-scores represent signal intensity above array background in standard deviation units, while S-scores reflect the specificity gap between the top-ranked target and subsequent proteins. An S-score greater than 2.5 is generally considered supportive of target-specific antibody recognition.

SDS-PAGE analysis of purified, BSA-free BMI1 antibody as confirmation of integrity and purity.