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Home >> Antibodies >> BiP Antibody / GRP78 / HSPA5

BiP Antibody / GRP78 / HSPA5 [clone BOO-8] (RQ5350)

  Catalog No Formulation Size Price (USD)  
Image RQ5350 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ul 429
Microvalidated Rab Mono Image
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IHC staining of FFPE human pancreas tissue with BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) LNCaP and 2) HepG2 cell lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name BOO-8
Purity Affinity purified
UniProt P11021
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This BiP antibody is available for research use only.
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Description

The protein encoded by the HSPA5 gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [RefSeq]

Application Notes

Optimal dilution of the BiP antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human HSPA5 / BiP was used as the immunogen for the BiP antibody.

Storage

Store the BiP antibody at -20oC.

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