BiP Antibody / GRP78 / HSPA5 (R30913)

BiP Antibody Human Liver Cancer IHC. IHC staining of FFPE human liver cancer tissue was performed with BiP Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. HRP-conjugated secondary antibody and DAB were used for detection. BiP staining is observed predominantly in the cytoplasm of tumor cells, with strong widespread staining throughout the cancer tissue. This BiP Antibody demonstrates localization of HSPA5/GRP78 in human liver cancer tissue.

BiP Antibody Human Cerebral Cortex IHC. IHC staining of FFPE human cerebral cortex tissue was performed with BiP Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. HRP-conjugated secondary antibody and DAB were used for detection. BiP staining is observed in neurons throughout the cerebral cortex, with prominent cytoplasmic staining in neuronal cell bodies and processes. This BiP Antibody demonstrates localization of HSPA5/GRP78 in human cerebral cortex tissue.

BiP Antibody Human, Mouse and Rat WB. Western blot analysis was performed with BiP Antibody on human U251, HepG2, K562 and A431 cell lysates (lanes 1-4), rat brain and liver tissue lysates (lanes 5-6), and mouse brain and liver tissue lysates (lanes 7-8). Samples were separated by 10% SDS-PAGE under reducing conditions, and the antibody was used at 0.17 ug/ml overnight at 4oC. A prominent band is detected at approximately 78 kDa, consistent with the expected migration of BiP/HSPA5/GRP78. This BiP Antibody demonstrates detection of HSPA5 across human, mouse and rat samples.

BiP Antibody Porcine Intestine WB. Western blot analysis was performed with BiP Antibody on lysates from six different segments of porcine large and small intestine (lanes 1-6). Samples were separated by 5-20% SDS-PAGE under reducing conditions, and the antibody was used at 1:2000 overnight at 4oC. A specific band is detected at approximately 70-78 kDa, consistent with the expected molecular weight of BiP/HSPA5/GRP78. Actin at approximately 42 kDa is shown as a loading control. This BiP Antibody demonstrates detection of HSPA5/GRP78 across multiple regions of porcine intestinal tissue.