BiP Antibody / Immunoglobulin Heavy Chain Binding Protein Antibody [clone BOO-8] (RQ5350)

BiP Antibody Human Pancreas IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of human pancreas tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates cytoplasmic expression throughout the pancreatic tissue, with strong signal in exocrine acinar cells and comparatively moderate staining within the pancreatic islet. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of secretory protein folding and endoplasmic reticulum homeostasis in human pancreas.

Western blot testing of human 1) LNCaP and 2) HepG2 cell lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.

BiP Antibody Human Thyroid Cancer IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of human thyroid cancer tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates variable cytoplasmic expression in malignant thyroid epithelial cells, including cells surrounding colloid-containing follicular structures, with comparatively limited stromal signal. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of endoplasmic reticulum stress and protein-folding demand in thyroid cancer.

BiP Antibody Human Non-Hodgkin Lymphoma IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of human non-Hodgkin lymphoma tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates widespread, variable cytoplasmic expression among the neoplastic lymphoid cells, with weak to moderate signal intensity across the tissue. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of secretory protein processing and endoplasmic reticulum stress in lymphoid malignancies.

BiP Antibody Human Prostate Cancer IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of human prostate cancer tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates weak to moderate cytoplasmic expression in malignant prostate epithelial cells, with variable intensity across the tumor and limited staining in the intervening stroma. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of protein-folding demand and endoplasmic reticulum stress in prostate cancer.

BiP Antibody Mouse Skin IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of mouse skin tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates cytoplasmic expression in epidermal keratinocytes and cells associated with cutaneous adnexal structures, with scattered signal in the underlying dermis. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of endoplasmic reticulum homeostasis and cellular stress responses in mouse skin.

BiP Antibody Rat Testis IHC. BiP was detected in a formalin-fixed, paraffin-embedded section of rat testis tissue. Heat-induced epitope retrieval was performed by boiling the section in 10 mM citrate buffer, pH 6.0, for 10–20 minutes followed by cooling at room temperature for 20 minutes. Staining with BiP antibody clone BOO-8 demonstrates diffuse cytoplasmic expression among developing germ cell populations within the seminiferous tubules, with comparatively limited interstitial staining. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports studies of secretory protein processing, endoplasmic reticulum homeostasis and stress responses in rat reproductive tissue.

BiP Antibody HepG2 Liver Cancer Cell IF. BiP was detected in formalin-fixed, paraffin-embedded human HepG2 liver cancer cells following heat-induced epitope retrieval by steaming in citrate buffer, pH 6.0, for 20 minutes. Immunofluorescent staining with BiP antibody clone BOO-8 shows the target protein in green, while nuclei were counterstained with DAPI and appear blue. BiP displays strong granular and reticular cytoplasmic staining with prominent perinuclear distribution, consistent with localization to the endoplasmic reticulum. This BiP Antibody, an immunoglobulin heavy chain binding protein antibody, supports cellular studies of protein folding and endoplasmic reticulum stress.

Western blot testing of human HEK293 cell lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.

Western blot testing of mouse liver tissue lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.