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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Prostate Specific Antigen Antibody Biotin Conjugate / PSA KLK3 Biotinylated Antibody [clone RM323] (R20347BTN)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20347BTN-0.05ML | Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 50 ul | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Biotin Conjugate |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM323 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P07288 |
| Applications | Immunohistochemistry (FFPE) : 1:500-1:1000 Western Blot (Non-reduced) : 1:1000-1:2500 ELISA : 1:100-1:5000 |
| Limitations | This biotinylated Prostate Specific Antigen antibody is available for research use only. |
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Prostate specific antigen (PSA), encoded by the KLK3 gene, is a secreted serine protease produced primarily by luminal epithelial cells of the prostate gland. PSA belongs to the kallikrein related peptidase family and functions in the proteolytic processing of seminal fluid proteins. Prostate Specific Antigen Antibody Biotin Conjugate enables sensitive detection of PSA in immunoassay systems that use biotin-streptavidin signal amplification, supporting studies examining prostate derived proteins and prostate cancer biomarkers.
Biotin conjugated antibodies are widely used in immunoassay workflows because biotin binds with extremely high affinity to streptavidin or avidin proteins. In ELISA and related immunodetection methods, a biotinylated antibody can function as a detection reagent that binds the target antigen while streptavidin conjugated enzymes or fluorophores generate the measurable signal. This amplification strategy allows highly sensitive detection of proteins present at low concentrations in biological samples.
PSA is secreted by prostate epithelial cells and is present in seminal plasma and at lower concentrations in blood and other biological fluids. Immunoassay detection of PSA is therefore commonly performed using sandwich ELISA formats in which one antibody captures PSA from the sample and a second antibody, such as a biotin conjugated PSA antibody, is used for detection. Following binding of the detection antibody, streptavidin conjugated enzymes such as alkaline phosphatase or horseradish peroxidase can be applied to generate a quantifiable signal.
Because PSA is highly specific to prostate epithelial tissue, detection of KLK3 protein is widely used in research examining prostate cancer biology, prostate epithelial differentiation, and secretion of prostate derived proteins. Immunoassay based measurement of PSA levels can also support studies investigating prostate tumor progression and prostate cell biology.
A recombinant rabbit monoclonal Prostate Specific Antigen Antibody Biotin Conjugate such as clone RM323 provides a convenient reagent for immunoassay detection of PSA. The antibody binds KLK3 protein while the biotin label enables interaction with streptavidin-based detection systems, supporting sensitive measurement of PSA in ELISA and other biotin dependent assay formats.
The stated application concentrations are suggested starting points. Titration of the recombinant Prostate Specific Antigen Antibody Biotin Conjugate may be required due to differences in protocols and secondary/substrate sensitivity.
Native PSA protein purified from human seminal fluid was used as the immunogen for the recombinant biotinylated Prostate Specific Antigen antibody.
Store the biotinylated Prostate Specific Antigen antibody at -20oC.
PSA antibody, KLK3 antibody, Kallikrein related peptidase 3 antibody, Human prostate specific antigen antibody
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