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Home >> Antibodies >> BHMT Antibody

BHMT Antibody (RQ6163)

  Catalog No Formulation Size Price (USD)  
Image RQ6163 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U937 cells with BHMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BHMT antibody.
Flow cytometry testing of human HepG2 cells with BHMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BHMT antibody.
Western blot testing of 1) rat liver, 2) mouse liver and 3) monkey liver lysate with BHMT antibody. Predicted molecular weight: ~45 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide
UniProt Q93088
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This BHMT antibody is available for research use only.
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    Reactivity : Human, Mouse, Rat
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Description

This gene encodes 'Betaine--homocysteine S-methyltransferase 1,' a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Application Notes

Optimal dilution of the BHMT antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids E80-Q406) was used as the immunogen for the BHMT antibody.

Storage

After reconstitution, the BHMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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