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Home >> Antibodies >> Betacellulin Antibody / BTC

Betacellulin Antibody / BTC (F54607)

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  Catalog No Formulation Size Price (USD)  
Image F54607-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54607-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human pancreas tissue with Betacellulin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human pancreas tissue with Betacellulin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MDA-MB-231 cells with Betacellulin antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MDA-MB-231 cell lysate with Betacellulin antibody. Expected molecular weight: 20-40 kDa depending on glycosylation level.
Flow cytometry testing of human HeLa cells with Betacellulin antibody; Blue=isotype control, Green= Betacellulin antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P35070
Applications Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Betacellulin antibody is available for research use only.
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Description

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Betacellulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 17-45 from the human protein was used as the immunogen for the Betacellulin antibody.

Storage

Aliquot the Betacellulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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