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Home >> Antibodies >> Beta Tubulin Antibody / TUBB

Beta Tubulin Antibody / TUBB [clone BI-20] (RQ5179)

  Catalog No Formulation Size Price (USD)  
Image RQ5179 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
Microvalidated Rab Mono Image
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IF/ICC staining of mouse NIH3T3 cells with Beta Tubulin antibody (red), PCNA antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human MCF7, 2) monkey COS-1, 3) human Jurkat and 4) human HeLa lysate with Beta Tubulin antibody. Predicted molecular weight: 50-55 kDa.
IHC staining of FFPE human uterine cancer with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human glioma with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name BI-20
Purity Affinity purified
UniProt P07437
Applications Western blot : 1:3000-1:10000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence/Immunocytochemistry : 1:50-1:200
Limitations This Beta Tubulin antibody is available for research use only.
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Description

The TUBB gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [RefSeq]

Application Notes

Optimal dilution of the Beta Tubulin antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Beta Tubulin / TUBB was used as the immunogen for the Beta Tubulin antibody.

Storage

Store the Beta Tubulin antibody at -20oC.

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