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Home >> Antibodies >> Beta-nerve growth factor Antibody / NGF

Beta-nerve growth factor Antibody / NGF (F54948)

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  Catalog No Formulation Size Price (USD)  
Image F54948-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54948-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with Beta-nerve growth factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with Beta-nerve growth factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MDA-MB-231 cells with Beta-nerve growth factor antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human NCI-H460 cell lysate with Beta-nerve growth factor antibody. Predicted molecular weight ~27 kDa.
Flow cytometry testing of human NCI-H460 cells with Beta-nerve growth factor antibody; Blue=isotype control, Green= Beta-nerve growth factor antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P01138
Localization Cytoplasmic, secreted
Applications Immunofluorescence : 1:10-1:50
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This Beta-nerve growth factor antibody is available for research use only.
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Description

NGFB is a member of the NGF-beta family. It is a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in the gene encoding NGFB have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the gene's expression is associated with allergic rhinitis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Beta-nerve growth factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 84-115 from the human protein was used as the immunogen for the Beta-nerve growth factor antibody.

Storage

Aliquot the Beta-nerve growth factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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