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Home >> Antibodies >> Beta Glucuronidase Antibody / Gusb

Beta Glucuronidase Antibody / Gusb (RQ5581)

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  Catalog No Formulation Size Price (USD)  
Image RQ5581 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human Caco-2 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
Flow cytometry testing of human A431 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
Western blot testing of human 1) K562, 2) Caco-2, 3) U-87 MG and 4) HepG2 cell lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P08236
Applications Western blot : 0.25-0.5ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 2-4ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Beta Glucuronidase antibody is available for research use only.
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Description

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

Application Notes

Optimal dilution of the Beta Glucuronidase antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids Q416-T651) was used as the immunogen for the Beta Glucuronidase antibody.

Storage

After reconstitution, the Beta Glucuronidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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