Beta-Catenin Polyclonal Antibody / CTNNB1 Polyclonal Antibody (R32807)

Beta-Catenin Polyclonal Antibody WB with CTNNB1 Knockout Validation. Western blot analysis of wild-type (WT) and CTNNB1 knockout (KO) human HEK293 whole cell lysates using the CTNNB1 Polyclonal Antibody at 0.5 ug/ml. Samples containing 30 ug of total protein per lane were separated by 10% SDS-PAGE under reducing conditions. A prominent band at approximately 95-100 kDa in WT cells is substantially reduced in CTNNB1 KO cells, supporting target-specific detection. The calculated molecular weight of beta-catenin is approximately 85 kDa, although migration near 95 kDa has also been reported with other commercially available antibodies. Beta-actin was used as a loading control and shows comparable signal in both samples.

Beta-Catenin Polyclonal Antibody A431 Cell IF. Immunofluorescence analysis of FFPE human A431 cells using Beta-Catenin antibody shows distinct membranous green fluorescence outlining cell borders, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with minimal cytoplasmic signal. DAPI nuclear stain (blue) highlights nuclei, providing contrast to the peripheral staining pattern. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody A549 Cell FACS. Flow cytometry analysis of human A549 cells using Beta-Catenin antibody shows a clear rightward shift of the blue population relative to both the red cells-only control and the green isotype control, indicating specific detection of CTNNB1 / Catenin beta-1 expression. The fluorescence profile supports intracellular localization consistent with beta-catenin distribution in cytoplasmic and membrane-associated pools following fixation and permeabilization. Red represents cells alone, green represents isotype control, and blue represents Beta-Catenin antibody staining.

Beta-Catenin Polyclonal Antibody Breast Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human breast cancer tissue using Beta-Catenin antibody shows strong membranous staining outlining tumor epithelial cells, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with mild cytoplasmic signal in select cells. Tumor glandular structures are clearly defined, while surrounding stromal regions display comparatively low staining. Hematoxylin counterstain provides nuclear contrast and tissue architecture. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Prostate Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human prostate cancer tissue using Beta-Catenin antibody shows strong membranous and cytoplasmic staining in tumor epithelial cells, consistent with CTNNB1 / Catenin beta-1 localization, with heterogeneous intensity across tumor regions. Malignant glandular structures are well delineated, while surrounding stromal tissue displays comparatively low staining. Hematoxylin counterstain provides clear nuclear contrast and architectural detail. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Liver Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human liver cancer tissue using CTNNB1 Polyclonal Antibody shows diffuse cytoplasmic and membranous staining in tumor cells, consistent with CTNNB1 / Catenin beta-1 localization, with variable intensity across the tumor field. Tumor architecture is preserved with clusters of malignant cells displaying moderate to strong signal, while surrounding non-tumor regions show comparatively lower staining. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Mouse Liver Tissue IHC. Immunohistochemistry analysis of FFPE mouse liver tissue using Beta-Catenin antibody shows prominent membranous staining outlining hepatocyte borders, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with mild cytoplasmic signal in hepatocytes. Hepatic architecture is well preserved, and non-parenchymal regions display comparatively lower staining intensity. Hematoxylin counterstain provides nuclear contrast and structural detail. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Mouse Small Intestine Tissue IHC. Immunohistochemistry analysis of FFPE mouse small intestine tissue using Beta-Catenin antibody shows strong membranous staining along epithelial cell borders lining the villi and crypts, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with mild cytoplasmic signal in enterocytes. Intestinal architecture is clearly preserved, and underlying stromal regions display comparatively low staining. Hematoxylin counterstain provides nuclear contrast and tissue context. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Rat Liver Tissue IHC. Immunohistochemistry analysis of FFPE rat liver tissue using Beta-Catenin antibody shows membranous staining outlining hepatocyte borders with additional cytoplasmic signal, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions and intracellular pools. Hepatic architecture is preserved, with hepatocyte plates clearly defined and non-parenchymal regions displaying comparatively low staining intensity. Hematoxylin counterstain provides nuclear contrast and structural detail. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Rat Small Intestine Tissue IHC. Immunohistochemistry analysis of FFPE rat small intestine tissue using Beta-Catenin antibody shows strong membranous staining along epithelial cell borders lining villi and crypt structures, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with mild cytoplasmic signal in enterocytes. Intestinal architecture is well preserved, and underlying stromal compartments display comparatively low staining intensity. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Rat Spleen Tissue IHC. Immunohistochemistry analysis of FFPE rat spleen tissue using Beta-Catenin antibody shows diffuse cytoplasmic and membranous staining across splenic cell populations, consistent with CTNNB1 / Catenin beta-1 localization, with variable intensity between red pulp and lymphoid regions. Cellular architecture is preserved, and background staining remains low in non-cellular areas. Hematoxylin counterstain provides clear nuclear contrast and tissue context. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Rat Heart Tissue IHC. Immunohistochemistry analysis of FFPE rat heart tissue using Beta-Catenin antibody shows membranous staining along cardiomyocyte cell borders, consistent with CTNNB1 / Catenin beta-1 localization at intercellular junctions, with low to moderate cytoplasmic signal. Cardiac muscle fibers are well preserved, and interstitial regions display minimal staining. Hematoxylin counterstain provides nuclear contrast and structural detail. HIER: steam FFPE sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

Beta-Catenin Polyclonal Antibody Human Cell Line Panel WB. Western blot analysis of 1) human placenta, 2) PC-3, 3) U-87 MG, 4) Caco-2, 5) HeLa, 6) A549, and 7) K562 lysates using Beta-Catenin Antibody for WB detects a consistent band at approximately 90-95 kDa across all samples, aligning with the predicted molecular weight of Catenin beta-1 / CTNNB1 at ~85 kDa with a characteristic upward shift during SDS-PAGE. The uniform banding pattern across diverse epithelial and cancer cell lines supports reliable detection of CTNNB1 and is consistent with phosphorylation-regulated electrophoretic mobility of beta-catenin.

Beta-Catenin Polyclonal Antibody Rodent Tissue WB. Western blot analysis of 1) rat heart, 2) mouse heart, 3) mouse lung, and 4) mouse liver lysates using Beta-Catenin Antibody for WB detects a consistent band at approximately 90-95 kDa across all samples, aligning with the predicted molecular weight of Catenin beta-1 / CTNNB1 at ~85 kDa with a characteristic upward shift during SDS-PAGE. The uniform banding pattern across rodent tissues supports cross-species detection of CTNNB1 and is consistent with phosphorylation-associated modulation of beta-catenin electrophoretic mobility.

Beta-Catenin Polyclonal Antibody Intestinal Cancer Tissue IF. Immunofluorescence analysis of FFPE human intestinal cancer tissue using CTNNB1 Polyclonal Antibody shows strong red fluorescence outlining epithelial cell borders with additional cytoplasmic signal, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions and intracellular pools. Tumor glandular architecture is clearly defined, and DAPI nuclear stain (blue) highlights nuclei, providing contrast to the beta-catenin signal. HIER: boil FFPE tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.