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Home >> Antibodies >> Beta Catenin Antibody

Beta Catenin Antibody (R30312)

  Catalog No Formulation Size Price (USD)  
Image R30312 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Immunofluorescent staining of FFPE human A431 cells with beta Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: beta Catenin antibody testing of human breast cancer tissue
Beta Catenin Antibody IHC-F. Immunohistochemistry staining of frozen rat intestine tissue with beta Catenin antibody.
Western blot testing of beta Catenin antibody and Lane 1: MM453; 2: MCF-7; 3: HeLa cell lysate. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P35222
Localization Cell surface, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Limitations This beta Catenin antibody is available for research use only.
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Description

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. Beta-catenin binds the cytoplasmic domain of some cadherins. It is an adherens junction protein that plays an important role in various aspects of liver biology.

This antibody complements our Beta-Catenin Antibody / CTNNB1 Antibody (clone CTNNB1/2030R) for broader analysis of CTNNB1 expression and localization.

Application Notes

Titration of the beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 764-781 (DGLPPGDSNQLAWFDTDL) were used as the immunogen for this beta Catenin antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the pAb can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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