Beta Catenin Polyclonal Antibody / CTNNB1 Antibody (R30312)

Beta Catenin Polyclonal Antibody by IP in Human A431 Cell Lysate. Immunoprecipitation of CTNNB1 from human A431 whole-cell lysate followed by Western blot analysis. Lane 1 contains 30 ug of A431 input lysate, lane 2 contains a rabbit IgG control immunoprecipitation, and lane 3 contains protein immunoprecipitated from 500 ug of A431 lysate using 2 ug of beta-catenin antibody. The immunoblot was probed with the rabbit polyclonal antibody at 0.5 ug/ml and detected using an HRP-conjugated secondary antibody. A band near 95 kDa is present in the input and antibody immunoprecipitation lanes, demonstrating enrichment of CTNNB1 relative to the control IgG sample.

Beta Catenin Polyclonal Antibody by WB in Eight Human Cell Lines. Western blot analysis of CTNNB1 in human HepG2 (lane 1), HeLa (lane 2), MCF-7 (lane 3), A431 (lane 4), RT4 (lane 5), SiHa (lane 6), T47D (lane 7), and THP-1 (lane 8) whole-cell lysates. Samples containing 30 ug of protein per lane were separated by 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. The rabbit polyclonal antibody was used at 0.5 ug/ml, followed by HRP-conjugated secondary antibody and chemiluminescent detection. A prominent band was detected at approximately 95 kDa across the eight samples, compared with the calculated molecular weight of 85.5 kDa for beta-catenin. The observed migration is consistent with the commonly reported apparent molecular weight of this protein.

Beta Catenin Polyclonal Antibody by WB in Rat and Mouse Tissues and Cell Lysates. Western blot analysis of CTNNB1 in rat brain (lane 1), rat liver (lane 2), rat C6 cells (lane 3), mouse brain (lane 4), and mouse liver (lane 5). Each lane contained 30 ug of protein separated by 10% SDS-PAGE under reducing conditions. The rabbit polyclonal antibody was applied at 0.5 ug/ml, followed by HRP-conjugated secondary antibody and chemiluminescent detection. A prominent band was detected near 95 kDa in all five samples, compared with the calculated molecular weight of approximately 85.5 kDa. These results demonstrate detection of beta-catenin in multiple rodent tissues and cells.

Beta Catenin Polyclonal Antibody by IHC in Human Endometrial Carcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue using rabbit polyclonal CTNNB1 antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. Detection was performed using an HRP-conjugated secondary detection system with DAB chromogen. The staining demonstrates beta-catenin expression in the carcinoma tissue, supporting investigation of CTNNB1 distribution in epithelial malignancies.