- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Beta-Catenin Antibody / CTNNB1 Literature-Validated Antibody [clone 5H10] (V3249)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3249-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
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V3249-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V3249SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 5H10 |
| Purity | Protein G affinity chromatography |
| UniProt | P35222 |
| Localization | Cell surface, cytoplasmic, cell junctions |
| Applications | Flow Cytometry : 0.5-1ug/million cells Immunofluorescence : 1-2ug/ml Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Beta Catenin antibody is available for research use only. |
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Catenin beta-1 (CTNNB1) is a multifunctional protein that plays essential roles in cell adhesion and Wnt signaling, making it one of the most widely studied targets in cell biology and cancer research. The Beta-Catenin Antibody / CTNNB1 Literature-Validated Antibody (clone 5H10) is associated with extensive use in peer-reviewed studies, supporting its utility for investigating CTNNB1 expression, localization, and regulation. CTNNB1 is encoded on chromosome 3p22.1 and belongs to the armadillo repeat protein family, which mediates protein-protein interactions with cadherins, transcription factors, and regulatory complexes.
The Beta-Catenin Antibody / CTNNB1 Literature-Validated Antibody, also referred to as CTNNB1 antibody and Catenin beta-1 antibody in the literature, recognizes a protein with dual functional roles in cellular structure and signaling. At the plasma membrane, beta-catenin associates with E-cadherin and other cadherins to stabilize adherens junctions and maintain epithelial integrity. In this context, CTNNB1 links cadherin complexes to the actin cytoskeleton through interactions with alpha-catenin, supporting tissue architecture and cell-cell adhesion.
In addition to its structural role, beta-catenin functions as a key mediator of canonical Wnt signaling. Under basal conditions, CTNNB1 is regulated by the destruction complex, which includes APC, AXIN, GSK3beta, and CK1, leading to phosphorylation and proteasomal degradation. Upon pathway activation, beta-catenin becomes stabilized and accumulates in the cytoplasm before translocating to the nucleus, where it interacts with TCF/LEF transcription factors to regulate gene expression. This dynamic regulation makes CTNNB1 a central node in pathways controlling proliferation, differentiation, and stem cell maintenance.
This Beta-Catenin Antibody / CTNNB1 Literature-Validated Antibody is uniquely positioned for studies requiring a well-characterized reagent with demonstrated performance across multiple experimental contexts. The extensive literature associated with clone 5H10 reflects its use in diverse applications, including analysis of subcellular localization, pathway activation, and tumor biology. Its widespread adoption supports reproducibility and comparability across studies, making it a valuable tool for both exploratory and confirmatory research.
Alterations in CTNNB1 are implicated in numerous cancers, including colorectal carcinoma, hepatocellular carcinoma, breast cancer, and melanoma. Mutations that disrupt beta-catenin degradation lead to protein accumulation and constitutive activation of Wnt signaling, contributing to tumor initiation and progression. As a result, beta-catenin antibody detection is frequently used to assess pathway dysregulation, cellular differentiation states, and disease-associated changes in protein distribution.
The mouse monoclonal clone 5H10 provides consistent detection of CTNNB1 across experimental systems, supporting studies that require reliable and widely referenced antibody performance. This antibody targets beta-catenin for research applications focused on understanding CTNNB1 function in adhesion, signaling, and disease. Its literature-backed usage enhances confidence in experimental interpretation and supports integration with previously published findings.
This antibody complements our Beta-Catenin Antibody / CTNNB1 Antibody (clone CTNNB1/2030R) for broader analysis of CTNNB1 expression and localization.
Optimal dilution of the Beta Catenin antibody to be determined by the researcher.
A C-terminal 100 amino acid segment from chicken beta-Catenin was used as the immunogen for the Beta Catenin antibody.
Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Beta-catenin validated antibody, CTNNB1 literature antibody, Catenin beta-1 antibody clone 5H10, Beta catenin published antibody, CTNNB1 research validated antibody
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