- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Beta-2 Microglobulin Antibody C21.48A1 / Free B2M Marker [clone C21.48A1] (V2803)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2803-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V2803-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V2803SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | C21.48A1 |
| Purity | Protein G affinity chromatography |
| UniProt | P61769 |
| Localization | Cytoplasmic |
| Applications | Flow Cytometry : 1-2ug/million cells Western Blot : 1-2ug/ml Immunofluorescence : 1-2ug/ml |
| Limitations | This Beta-2 Microglobulin Antibody C21.48A1 / Free B2M Marker is available for research use only. |
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Beta-2 Microglobulin Antibody C21.48A1 / Free B2M Marker detects beta-2 microglobulin (B2M), with clone C21.48A1 recognizing a hidden determinant that becomes available when beta-2 microglobulin is separated from the HLA heavy chain. This distinctive recognition differentiates C21.48A1 from antibodies directed against determinants accessible on HLA-associated B2M. Beta-2 microglobulin is a small protein that normally associates noncovalently with major histocompatibility complex class I heavy chains and is essential to the structure and function of MHC class I complexes.
Within MHC class I complexes, B2M supports proper folding, stability and cell-surface expression of the HLA heavy chain. These complexes present intracellularly derived peptide antigens to CD8-positive T cells and thereby contribute to adaptive immune recognition and surveillance. Beta-2 microglobulin itself does not span the plasma membrane and can dissociate from the MHC class I heavy chain, producing free B2M in tissues and biological fluids.
Free and soluble beta-2 microglobulin are investigated in a variety of physiological and disease settings. Serum B2M levels reflect a combination of cellular production, turnover and renal clearance and may increase during immune activation, inflammation and infection. Elevated levels are also associated with several hematologic malignancies and renal disorders. Urinary beta-2 microglobulin is studied in relation to proximal renal tubular function and conditions affecting renal handling of the protein.
Beta-2 microglobulin is also relevant to cancer and immunology research through its relationship with MHC class I antigen presentation. Alterations affecting B2M expression or its association with HLA class I can influence immune recognition of cells. The ability to investigate different structural states or determinants of B2M can therefore complement studies using antibodies directed more broadly against beta-2 microglobulin and MHC class I-associated protein.
Clone C21.48A1 is a mouse monoclonal antibody generated using soluble beta-2 microglobulin as the immunogen. It recognizes a hidden B2M determinant exposed when beta-2 microglobulin is separated from the HLA heavy chain and has been evaluated by Western blot, immunofluorescence and flow cytometry in human cells. NSJ Bioreagents supplies antibodies for immunology and antigen-presentation research. A Beta-2 Microglobulin Antibody can be used to investigate B2M expression, free beta-2 microglobulin and the relationship between B2M and HLA class I biology.
For additional research on beta-2 microglobulin expression, MHC class I complexes and antigen presentation, including a protein microarray validated reagent, see our Beta-2 Microglobulin Antibody / MHC Class I Component Antibody page.
Optimal dilution of the Beta-2 Microglobulin Antibody C21.48A1 / Free B2M Marker should be determined by the researcher.
Soluble beta 2 microglobulin was used as the immunogen for the Beta-2 Microglobulin Antibody C21.48A1.
Store the Beta-2 Microglobulin Antibody C21.48A1 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Beta-2 Microglobulin antibody, Free B2M Marker antibody, B2M antibody, Beta-2-Microglobulin antibody, Free Beta-2 Microglobulin antibody, C21.48A1 antibody
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