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Home >> Antibodies >> Beclin 1 Antibody

Beclin 1 Antibody (F46279)

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  Catalog No Formulation Size Price (USD)  
Image F46279-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F46279-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of Beclin 1 antibody and 293 lysate transiently transfected with the BECN1 gene (2ug/lane).
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Western blot analysis of Beclin 1 antibody and 293 lysate transiently transfected with the BECN1 gene (2ug/lane).
The Beclin 1 antibody used in western blot to detect BECN1 in mouse liver tissue lysate
IHC analysis of FFPE human breast carcinoma tissue stained with the Beclin 1 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Chicken
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q14457
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This Beclin 1 antibody is available for research use only.
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Description

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Application Notes

Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 181-210 from the human protein was used as the immunogen for this Beclin 1 antibody.

Storage

Aliquot the Beclin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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