• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> BDNF Antibody

BDNF Antibody (R31503)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R31503 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Western blot testing of BDNF antibody (0.5ug/ml) and rat brain lysate. Expected molecular weight: 27~37 kDa.
›
Western blot testing of BDNF antibody (0.5ug/ml) and rat brain lysate. Expected molecular weight: 27~37 kDa.
IHC-P testing of mouse brain tissue. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC-P testing of rat brain tissue. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human U-87 MG cells with BDNF antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=BDNF antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P23560
Localization Cytoplasmic, secreted
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This BDNF antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB
    Reactivity : Human, Mouse, Rat
  • Applications : FACS, WB, IHC, IF, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine, Pig
  • Applications : IF, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Dog

Description

Brain-derived neurotrophic factor is a secreted protein that, in humans, is encoded by the BDNF gene. It is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. It has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Application Notes

The stated application concentrations are suggested starting points. Titration of the BDNF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 129-247) was used as the immunogen for this BDNF antibody.

Storage

After reconstitution, the BDNF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all BDNF antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.