BCOR Antibody / BCL-6 corepressor [clone BCOR/1311] (V5338)

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using BCOR antibody (clone BCOR/1311). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Immunohistochemistry analysis of BCOR antibody in human tonsil tissue. Formalin-fixed, paraffin-embedded human tonsil tissue stained with BCOR antibody (clone BCOR/1311) shows nuclear and perinuclear HRP-DAB brown staining in lymphoid cells, consistent with the expected subcellular localization of BCL-6 corepressor in proliferating germinal center regions. Background stromal elements show minimal staining. The inset image represents the negative control in which PBS was used in place of the primary antibody, demonstrating absence of specific brown chromogenic signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free BCOR antibody (clone BCOR/1311) as confirmation of integrity and purity.