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Home >> Antibodies >> Bcl6 Antibody

Bcl6 Antibody [clone PCRP-BCL6-1E2] (V9623)

  Catalog No Formulation Size Price (USD)  
Image V9623-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9623-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9623SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human MCF-7 cells using Bcl6 antibody (green, clone PCRP-BCL6-1E2) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Bcl6 antibody (blue, clone PCRP-BCL6-1E2), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Bcl6 antibody (clone PCRP-BCL6-1E2). These results demonstrate the foremost specificity of the PCRP-BCL6-1E2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name PCRP-BCL6-1E2
Purity Protein A/G affinity
UniProt P41182
Localization Nucleus, Cytoplasm
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This Bcl6 antibody is available for research use only.
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Description

Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( LH ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Application Notes

Optimal dilution of the Bcl6 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Bcl6 antibody.

Storage

Aliquot the Bcl6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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