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Home >> Antibodies >> BCL3 Antibody

BCL3 Antibody (RQ6314)

  Catalog No Formulation Size Price (USD)  
Image RQ6314 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse Neuro-2a, 4) human HEK293, 5) human Jurkat, 6) human PC-3 and 7) human HeLa cell lysate with BCL3 antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of human U-251 cells with BCL3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BCL3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P20749
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This BCL3 antibody is available for research use only.
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Description

B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. The BCL3 gene is mapped to human chromosome 19. This protein functions as a transcriptional coactivator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.

Application Notes

Optimal dilution of the BCL3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E118-Y307) was used as the immunogen for the BCL3 antibody.

Storage

After reconstitution, the BCL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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