BCL2L1 Antibody / Bcl-XL [clone 31B78] (FY13325)

BCL2L1 Antibody WB. Western blot analysis of human HeLa, Jurkat, HepG2, MCF7, and SH-SY5Y cell lysates using BCL2L1 Antibody demonstrates a prominent immunoreactive band at approximately 26-32 kDa, consistent with expression of BCL2L1/Bcl-XL. Bcl-XL is the major anti-apoptotic isoform encoded by the BCL2L1 gene and functions as a key regulator of mitochondrial apoptosis by inhibiting pro-apoptotic BCL2 family proteins and promoting cellular survival. A faint band near 50 kDa is observed in some cell lines and may represent an SDS-resistant Bcl-XL dimer or higher-order protein complex. This higher molecular weight species is substantially less abundant than the predominant monomeric form. Antibody incubation was performed at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight of Bcl-XL: approximately 26-30 kDa.

BCL2L1 Antibody Hematopoietic Cell WB. Western blot analysis of human Ramos (lane 1) and K562 (lane 2) cell lysates using BCL2L1 Antibody demonstrates distinct immunoreactive bands at approximately 26-32 kDa, consistent with expression of Bcl-XL, the major anti-apoptotic isoform encoded by the BCL2L1 gene. Bcl-XL functions as a critical regulator of mitochondrial apoptosis by inhibiting activation of pro-apoptotic BCL2 family proteins and promoting cellular survival under conditions of physiological and cellular stress. The observed banding pattern is consistent with endogenous BCL2L1 expression in hematopoietic-derived cell lines and supports specific detection of the protein by western blot analysis. Predicted molecular weight: approximately 26 kDa. Commonly observed molecular weight: 26-32 kDa.

BCL2L1 Antibody Cross-Species WB. Western blot analysis of mouse heart, mouse liver, mouse spleen, mouse lung, mouse skin, rat heart, and rat liver tissue lysates using BCL2L1 Antibody demonstrates a predominant immunoreactive band at approximately 26-32 kDa, consistent with expression of Bcl-XL, the major anti-apoptotic isoform encoded by the BCL2L1 gene. Bcl-XL functions as a critical regulator of mitochondrial apoptosis by inhibiting pro-apoptotic BCL2 family proteins and promoting cellular survival across diverse tissues. A faint band near 50 kDa is observed in selected samples and may represent an SDS-resistant Bcl-XL dimer or higher-order protein complex. This higher molecular weight species is substantially less abundant than the predominant monomeric form. Antibody incubation was performed at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight: approximately 26 kDa. Commonly observed molecular weight: 26-32 kDa.

BCL2L1 Antibody Brain Tissue WB. Western blot analysis of mouse brain and rat brain tissue lysates using BCL2L1 Antibody demonstrates prominent immunoreactive bands at approximately 26-32 kDa, consistent with expression of Bcl-XL, the major anti-apoptotic isoform encoded by the BCL2L1 gene. Bcl-XL is highly expressed in neuronal tissues where it contributes to mitochondrial integrity, neuronal survival, and protection against apoptosis-induced cell death. The observed banding pattern supports conserved BCL2L1 expression across rodent brain tissues and is consistent with the established role of Bcl-XL in maintaining survival of post-mitotic neuronal populations. Antibody incubation was performed at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight: approximately 26 kDa. Commonly observed molecular weight: 26-32 kDa.