Bcl-XS Antibody (R30334)

Bcl-XS Antibody WB. Western blot analysis of human HeLa (lane 1) and rat PC-12 (lane 2) cell lysates using Bcl-XS Antibody demonstrates distinct immunoreactive bands at approximately 19 kDa, consistent with expression of Bcl-XS, the pro-apoptotic short isoform generated by alternative splicing of the BCL2L1 gene. In contrast to the anti-apoptotic Bcl-XL isoform, Bcl-XS promotes apoptosis by antagonizing pro-survival BCL2 family proteins and facilitating activation of intrinsic cell death pathways. The observed cross-species reactivity supports detection of Bcl-XS in both human and rat samples. Predicted and observed molecular weight: approximately 19 kDa.

Bcl-XS Antibody Colon Cancer IHC. Immunohistochemistry staining of FFPE human colon cancer tissue using Bcl-XS Antibody demonstrates cytoplasmic HRP-DAB brown staining within malignant epithelial cells and scattered stromal-associated cellular populations. The staining pattern is consistent with expression of Bcl-XS, the pro-apoptotic short isoform generated by alternative splicing of the BCL2L1 gene. Unlike the anti-apoptotic Bcl-XL isoform, Bcl-XS promotes programmed cell death by antagonizing pro-survival BCL2 family proteins and facilitating activation of intrinsic apoptosis pathways. Expression of Bcl-XS is frequently investigated in studies of apoptosis regulation, tumor progression, and cellular responses to stress and therapeutic intervention. HIER was performed by steaming tissue sections in pH 6 citrate buffer prior to immunostaining.