Bcl-XL Antibody / Anti-Apoptotic Protein Antibody (R32762)

Bcl-XL Antibody FACS. Flow cytometry analysis of fixed and permeabilized human Jurkat cells using Bcl-XL Antibody / Anti-Apoptotic Protein Antibody demonstrates a pronounced rightward shift of the antibody-stained population (blue) relative to both the isotype control (green) and unstained cells (red), consistent with intracellular expression of Bcl-XL. Bcl-XL, encoded by the BCL2L1 gene, is a key anti-apoptotic member of the BCL2 protein family that promotes cellular survival by inhibiting mitochondrial apoptosis pathways and suppressing activation of pro-apoptotic signaling proteins. The observed staining pattern supports detection of Bcl-XL expression in Jurkat cells by flow cytometry. Cells were fixed, permeabilized, and stained with Bcl-XL antibody at 1 ug/million cells following blocking with goat sera. Red = cells alone, Green = isotype control, Blue = Bcl-XL antibody.

Bcl-XL Antibody WB. Western blot analysis of human Jurkat (lane 1), HeLa (lane 2), A549 (lane 3), and Raji (lane 4) cell lysates, as well as rat brain (lane 5), rat C6 (lane 6), mouse brain (lane 7), and mouse NIH 3T3 (lane 8) lysates using Bcl-XL Antibody / Anti-Apoptotic Protein Antibody demonstrates a distinct immunoreactive band at approximately 26-30 kDa across human, rat, and mouse samples. The detected band is consistent with expression of Bcl-XL, the major anti-apoptotic isoform encoded by the BCL2L1 gene. Bcl-XL functions at the outer mitochondrial membrane where it inhibits apoptosis by suppressing activation of pro-apoptotic BCL2 family proteins and preventing cytochrome c release. The broad cross-species reactivity observed supports the conserved role of Bcl-XL in regulating cell survival and mitochondrial integrity. Antibody concentration: 0.5 ug/ml. Predicted molecular weight: 26-30 kDa.

Bcl-XL Antibody IF. Immunofluorescent staining of FFPE human TPC-1 cells using Bcl-XL Antibody / Anti-Apoptotic Protein Antibody demonstrates diffuse cytoplasmic green fluorescence consistent with expression of Bcl-XL, the major anti-apoptotic protein encoded by the BCL2L1 gene. The staining pattern is compatible with the known intracellular localization of Bcl-XL, which functions primarily at mitochondrial and intracellular membrane compartments to suppress apoptosis and promote cellular survival. Bcl-XL plays a critical role in regulating mitochondrial integrity, inhibiting pro-apoptotic signaling pathways, and maintaining resistance to programmed cell death. Nuclei are counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining.

Bcl-XL Antibody Pancreatic Cancer IHC. Immunohistochemistry staining of FFPE human pancreatic cancer tissue using Bcl-XL Antibody / Anti-Apoptotic Protein Antibody demonstrates cytoplasmic and membranous HRP-DAB brown staining within malignant epithelial cells forming glandular tumor structures. The staining pattern is consistent with expression of Bcl-XL, the major anti-apoptotic protein encoded by the BCL2L1 gene. Bcl-XL functions as a key regulator of mitochondrial apoptosis by inhibiting pro-apoptotic BCL2 family members and promoting cellular survival under conditions of stress. Expression of Bcl-XL is frequently investigated in cancer biology due to its association with tumor cell survival, apoptosis resistance, and therapeutic response. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunostaining. HRP-conjugated secondary antibody and DAB chromogen were used for signal detection.

Bcl-XL Antibody IP. Immunoprecipitation analysis of Bcl-XL from human A549 whole cell lysate using Bcl-XL Antibody / Anti-Apoptotic Protein Antibody demonstrates specific enrichment of BCL2L1 following antibody-mediated pull-down. Bcl-XL is detected as a distinct band at approximately 26-30 kDa in the immunoprecipitated sample, consistent with the expected molecular weight of the major anti-apoptotic BCL2L1 isoform. The result confirms specific recognition and capture of endogenous Bcl-XL protein from cellular lysates. Bcl-XL functions as a key regulator of mitochondrial apoptosis, promoting cell survival through inhibition of pro-apoptotic BCL2 family members and preservation of mitochondrial integrity. Immunoprecipitation was performed using 2 ug of Bcl-XL antibody and 500 ug of human A549 whole cell lysate. The IgG heavy chain is visible at approximately 50 kDa.