BCL-6 corepressor Antibody / BCOR [clone BCOR/2372] (V5339)

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using BCL-6 corepressor antibody (clone BCOR/2372). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Immunohistochemistry analysis of BCL-6 corepressor antibody in human tonsil tissue (clone BCOR/2372). FFPE human tonsil tissue shows strong nuclear HRP-DAB brown staining in lymphoid cells, consistent with nuclear localization of BCL-6 corepressor / BCOR in proliferating and activated lymphoid populations. Staining is predominantly nuclear with minimal background in surrounding stromal elements. The inset image represents the negative control in which PBS was used instead of the primary antibody, demonstrating absence of specific nuclear staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free BCL-6 corepressor antibody (clone BCOR/2372) as confirmation of integrity and purity.