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Home >> Antibodies >> Bcl-2 Antibody

Bcl-2 Antibody [clone Bcl-2-100] (R30004)

  Catalog No Formulation Size Price (USD)  
Image R30004 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 329
Western blot testing of Bcl-2 antibody and rat tissue: 1. heart; 2. spleen; 3. small intestine; 4: liver. Predicted molecular weight ~26 kDa.
IHC testing of FFPE human breast cancer tissue with Bcl-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with Bcl-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name Bcl-2-100
Purity Ascites
Gene ID 596
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml in formalin/acetone fixed tissues
Immunocytochemistry : Suitable
Limitations This Bcl-2 antibody is available for research use only.
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Description

Immunoreactive BCL2 protein was detected in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of the protein, regardless of whether the breakpoint was located in or at a distance from the gene. Overexpression blocks the apoptotic death of a pro-B-lymphocyte cell line.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 41-54 of the human protein were used as the immunogen for this Bcl-2 antibody.

Storage

After reconstitution, the Bcl-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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