Bax Antibody / BCL2 Associated X Protein Antibody (R30183)

Bax Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue with Bax antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Bax immunoreactivity is observed predominantly within the cytoplasm of malignant hepatocytes, consistent with the expected localization of BCL2 Associated X Protein, a pro-apoptotic BCL2 family member that translocates to the mitochondrial outer membrane following cellular stress to initiate the intrinsic apoptotic pathway. Cell nuclei are counterstained with hematoxylin (blue).

Bax Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue with Bax antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Bax immunoreactivity is observed predominantly within the cytoplasm of lymphoid cells, consistent with the expected localization of this apoptosis regulator and pro-apoptotic BCL2 family protein that promotes mitochondrial outer membrane permeabilization and programmed cell death in response to cellular stress. Cell nuclei are counterstained with hematoxylin (blue).

Bax Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian cancer tissue with Bax antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Bax immunoreactivity is observed predominantly within the cytoplasm of malignant epithelial cells, consistent with the expected localization of BCL2 Associated X Protein, a pro-apoptotic member of the BCL2 family that regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway. Cell nuclei are counterstained with hematoxylin (blue).

Bax Antibody A549 Cell IF. Immunofluorescent staining of A549 cells with Bax antibody (green) at 5 ug/ml. Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Nuclei are counterstained with DAPI (blue). Bax immunoreactivity is observed predominantly within the cytoplasm, consistent with the expected localization of BCL2 Associated X Protein, a pro-apoptotic BCL2 family member that undergoes stress-induced translocation to the mitochondrial outer membrane to initiate the intrinsic apoptotic pathway.

Bax Antibody Human, Rat and Mouse WB. Western blot testing of 1) human THP-1, 2) human MCF-7, 3) human A549, 4) human HeLa, 5) rat brain, 6) rat C6, 7) mouse brain, and 8) mouse Neuro-2a lysates with Bax antibody at 0.5 ug/ml. A distinct immunoreactive band is detected at approximately 21 kDa in all samples, consistent with the predicted molecular weight of Bax. The results demonstrate specific detection of BCL2 Associated X Protein, a pro-apoptotic BCL2 family member that regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway following cellular stress.

Bax Antibody HeLa Wild-Type and Knockout WB. Western blot testing of 1) human HeLa wild-type (WT) and 2) human HeLa Bax knockout (KO) cell lysates with Bax antibody at 0.5 ug/ml. A specific immunoreactive band is detected at approximately 21 kDa in the wild-type lysate but is absent from the Bax knockout lysate, confirming specific detection of BCL2 Associated X Protein. Beta-actin serves as the loading control. These results provide genetic validation of antibody specificity for western blot analysis.

Bax Antibody HeLa Cell IP. Immunoprecipitation of endogenous BCL2 Associated X Protein (Bax) from human HeLa whole cell lysate using Bax antibody. Lane 1: Input HeLa whole cell lysate (30 ug). Lane 2: Rabbit control IgG immunoprecipitation (negative control). Lane 3: Immunoprecipitation with 2 ug Bax antibody and 500 ug HeLa whole cell lysate. Immunoprecipitated proteins were analyzed by western blot using the same antibody. A specific band is detected at approximately 21 kDa, consistent with the predicted molecular weight of BCL2 Associated X Protein, demonstrating efficient and specific immunoprecipitation of this pro-apoptotic BCL2 family protein.

Bax Antibody MCF-7 Cell FACS. Flow cytometric analysis of fixed and permeabilized human MCF-7 cells stained with Bax antibody. Red: blank control. Green: rabbit IgG isotype control. Blue: Bax antibody. The pronounced rightward shift of the Bax antibody-stained population relative to the isotype control demonstrates specific intracellular detection of BCL2 Associated X Protein (Bax), a pro-apoptotic BCL2 family protein that regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway following cellular stress.