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Home >> Antibodies >> Bax Antibody

Bax Antibody [clone BAX/962] (V2827)

  Catalog No Formulation Size Price (USD)  
Image V2827-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2827-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2827SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2827IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bax antibody (clone BAX/962).
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Bax antibody (clone BAX/962).
Flow cytometry testing of human Jurkat cells with Bax antibody (clone BAX/962); Red=isotype control, Blue= Bax antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name BAX/962
Purity Protein G affinity chromatography
UniProt Q07812
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Bax antibody is available for research use only.
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Description

Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Application Notes

Optimal dilution of the Bax antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Bax antibody.

Storage

Store the Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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