Bax Antibody / Apoptosis Regulator Antibody [clone 2D2] (V2005)

Description

Bax Antibody recognizes BCL2 Associated X Protein (Bax), a pro-apoptotic member of the BCL2 protein family that serves as a central regulator of the intrinsic, or mitochondrial, apoptotic pathway. Under normal conditions, Bax is predominantly localized within the cytosol as an inactive monomer. In response to cellular stress, DNA damage, oxidative injury, oncogene activation, or growth factor withdrawal, Bax undergoes conformational activation and translocates to the mitochondrial outer membrane, where it oligomerizes to promote membrane permeabilization. This process triggers the release of cytochrome c and other apoptogenic factors, leading to caspase activation and programmed cell death. Consequently, Bax Antibody is widely used to investigate apoptosis, mitochondrial biology, cancer, neurodegeneration, and therapeutic responses.

Bax functions in close coordination with other BCL2 family members to determine cellular fate. Anti-apoptotic proteins such as BCL2 and BCL-XL inhibit Bax activation, whereas BH3-only proteins including BID, BIM, and PUMA promote its conformational change and mitochondrial translocation. The balance between these pro- and anti-apoptotic regulators governs mitochondrial integrity and cell survival. Because Bax occupies a pivotal position within this signaling network, Bax Antibody has become an indispensable research tool for studies of apoptosis, chemotherapy sensitivity, radiation responses, ischemic injury, and developmental biology.

Altered Bax expression or activity has been implicated in numerous diseases, including leukemia, lymphoma, breast cancer, colorectal cancer, lung cancer, prostate cancer, hepatocellular carcinoma, Alzheimer's disease, Parkinson's disease, myocardial infarction, stroke, and autoimmune disorders. Reduced Bax activity may allow damaged cells to evade apoptosis and promote tumor progression, whereas excessive Bax activation contributes to inappropriate cell loss during neurodegeneration and tissue injury. Bax is therefore widely investigated as both a prognostic biomarker and a potential therapeutic target, particularly in combination with emerging BH3 mimetics and other apoptosis-modulating agents.

This mouse monoclonal antibody, clone 2D2, has been cited in more than 100 peer-reviewed scientific publications, demonstrating its widespread acceptance and reliability within the research community. NSJ Bioreagents offers this highly validated Bax Antibody for dependable detection of endogenous Bax in human and animal samples. It provides excellent performance across western blotting, immunohistochemistry, immunofluorescence, flow cytometry, immunoprecipitation, and related applications supporting studies of apoptosis, mitochondrial signaling, cancer biology, and cell death mechanisms.

Learn more about proteins that regulate programmed cell death, mitochondrial apoptosis and cell survival on our Apoptosis Antibodies page.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax Antibody / Apoptosis Regulator Antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Storage

Store the Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

BCL2 associated XBCL2 Associated X Protein antibody, BCL2 Like 4 antibody, BCL2L4 antibody, Bax antibody, BAX Protein antibody, BCL2 Associated X antibody