Basic Cytokeratin Antibody AE3 | V2329 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2329-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2329-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2329SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2329IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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Basic Cytokeratin antibody AE3 immunohistochemistry skin

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IHC: FFPE skin stained with Basic Cytokeratin antibody AE3.

SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone AE3). Confirmation of Integrity and Purity of the Antibody.

Species Reactivity	Human, Mouse, Rat
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	AE3
Purity	Protein G purified
Buffer	1X PBS, pH 7.4
Gene ID	3848
Localization	Cytoplasmic
Applications	Flow cytometry : 0.5-1ug/million cells Immunofluorescence : 1-2ug/ml Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1) Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations	This Basic Cytokeratin antibody AE3 is available for research use only. The formulation is not suitable for therapeutic or diagnostic testing.
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Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (Cat No V2330).

Application Notes

Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.