Basement Membrane Protein 40 Antibody / SPARC [clone r15G12] (V6002)

Western blot analysis of Basement Membrane Protein 40/SPARC antibody (clone r15G12) in human samples. Lysates from human brain tissue and human U87 glioblastoma cells show a distinct immunoreactive band at approximately 40-45 kDa, consistent with the predicted molecular weight of Secreted protein acidic and rich in cysteine. SPARC, also known as BM-40, is a secreted extracellular matrix protein that can exhibit slight mobility shifts on SDS-PAGE due to post-translational modifications such as glycosylation and calcium-binding related conformational properties. The observed band pattern supports detection of SPARC in both neural tissue and glioma-derived cells, consistent with reported extracellular matrix and stromal-associated expression.

Immunohistochemistry analysis of Basement Membrane Protein 40/SPARC antibody in human testis tissue (clone r15G12). FFPE human testis sections show HRP-DAB brown cytoplasmic and extracellular staining within seminiferous tubules, with prominent signal in germ cells and along the surrounding stromal matrix. The staining pattern highlights matrix-associated localization consistent with Secreted protein acidic and rich in cysteine expression. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, demonstrating absence of specific brown staining. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.