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Home >> Antibodies >> BAP1 Antibody

BAP1 Antibody (F47995)

  Catalog No Formulation Size Price (USD)  
Image F47995-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F47995-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of BAP1 antibody and mouse testis lysate. Expected molecular weight: 80-100 kDa.
IHC analysis of FFPE human breast carcinoma tissue stained with the BAP1 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat, Zebrafish
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q92560
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This BAP1 antibody is available for research use only.
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Description

'BRCA1-associated protein-1,' or BAP1 interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals.. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns.1 BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4.8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21.3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas.1 Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.1

Application Notes

Titration of the BAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 36-66 from the human protein was used as the immunogen for this BAP1 antibody.

Storage

Aliquot the BAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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