Band 3 Antibody / Anion Exchange Protein 1 Antibody (R31868)

Band 3 Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placenta tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within placental trophoblast cell populations. Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that mediates transmembrane ion transport and contributes to maintenance of acid-base balance and cellular ionic homeostasis. In addition to its well-established role in erythrocyte membrane physiology, Band 3 family proteins participate in transport processes that support cellular metabolism and physiologic homeostasis in specialized epithelial tissues. The observed placental staining pattern is consistent with expression of anion transport machinery involved in regulation of bicarbonate and chloride exchange. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

Band 3 Antibody Human Placenta Frozen IHC. Immunohistochemical staining of frozen human placenta tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates distinct membranous and cytoplasmic staining within placental trophoblast cell populations. Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that regulates transmembrane ion transport and contributes to maintenance of cellular acid-base balance and ionic homeostasis. While best known as a major erythrocyte membrane protein, AE1-related transport activity is also associated with specialized epithelial cells that require tightly regulated bicarbonate and chloride exchange. The observed trophoblast-associated staining pattern is consistent with expression of membrane transport proteins involved in placental physiology, ion balance, and cellular homeostasis.

Band 3 Antibody Mouse Kidney IHC. Immunohistochemical staining of FFPE mouse kidney tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells. Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that plays an important role in bicarbonate transport, acid-base regulation, and maintenance of cellular ionic homeostasis. In the kidney, AE1 contributes to physiologic mechanisms that regulate systemic acid-base balance through coordinated ion exchange and bicarbonate handling. The observed tubular staining pattern is consistent with the established role of Anion Exchange Protein 1 in renal epithelial transport processes and kidney physiology. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

Band 3 Antibody Rodent Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells. Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that plays a critical role in bicarbonate transport, acid-base regulation, and maintenance of cellular ionic homeostasis. In addition to its well-established function in erythrocyte membranes, AE1 is expressed in specialized renal epithelial cells where it contributes to physiologic acid-base balance through regulation of bicarbonate and chloride exchange. The observed tubular staining pattern is consistent with the established role of Band 3 family proteins in kidney ion transport and maintenance of renal homeostasis. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

Band 3 Antibody Human Placenta IF. Immunofluorescent staining of FFPE human placenta tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates strong green fluorescence within placental trophoblast cell populations, while nuclei are counterstained with DAPI (blue). Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that mediates transmembrane ion transport and contributes to maintenance of cellular acid-base balance and ionic homeostasis. Although best known as a major erythrocyte membrane protein, AE1-related transport mechanisms are also associated with specialized epithelial cells that require regulated bicarbonate and chloride exchange. The observed trophoblast-associated fluorescence pattern is consistent with expression of anion transport proteins involved in placental physiology, membrane transport, and cellular homeostasis. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

Band 3 Antibody Renal Tubule IF. Immunofluorescent staining of FFPE rat kidney tissue using Band 3 Antibody / Anion Exchange Protein 1 Antibody demonstrates strong green fluorescence within renal tubular epithelial structures, while nuclei are counterstained with DAPI (blue). Band 3 (AE1/SLC4A1) is a chloride/bicarbonate exchanger that plays a critical role in bicarbonate transport, acid-base regulation, and maintenance of cellular ionic homeostasis. In the kidney, AE1 is expressed in specialized epithelial cell populations where it contributes to physiologic mechanisms responsible for systemic acid-base balance and bicarbonate handling. The observed tubular fluorescence pattern is consistent with the established role of Anion Exchange Protein 1 in renal ion transport and epithelial homeostasis. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.