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Home >> Antibodies >> Band 3 Antibody / SLC4A1

Band 3 Antibody / SLC4A1 (R31868)

  Catalog No Formulation Size Price (USD)  
Image R31868 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of rat 1) brain, 2) kidney, 3) liver, 4) spleen and 5) human placenta with Band 3 antibody. Expected/observed molecular weight ~102 kDa.
IHC testing of FFPE human placenta with Band 3 antbody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placenta with Band 3 antbody.
IHC testing of FFPE mouse kidney with Band 3 antbody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat kidney with Band 3 antbody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Immunofluorescent testing of FFPE human placenta with Band 3 antbody (green) and DAPI nuclear stain (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Immunofluorescent testing of FFPE rat kidney with Band 3 antbody (green) and DAPI nuclear stain (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P02730
Localization Cytoplasmic, membrane
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 1-2ug/ml
Limitations This Band 3 antibody is available for research use only.
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Description

Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

Application Notes

Optimal dilution of the Band 3 antibody should be determined by the researcher.

Immunogen

Amino acids 28-365 of human Band 3 were used as the immunogen for the Band 3 antibody.

Storage

After reconstitution, the Band 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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