BAG6 Antibody / BAT3 (RQ4907)

Western blot testing of 1) human HeLa, 2) human ThP-1, 3) human U-87 MG, 4) rat brain, 5) rat heart, 6) rat lung, 7) rat liver, 8) mouse brain, 9) mouse heart and 10) mouse lung lysate with BAG6 antibody at 0.5ug/ml. Predicted molecular weight ~119 kDa but observed at 150-170 kDa.

Western blot testing of 1) human HeLa, 2) human U-87 MG, 3) human SH-SY5Y, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse Neuro-2a cell lysate with BAG6 antibody. Predicted molecular weight ~119 kDa but observed at 150-170 kDa.

Immunofluorescent staining of FFPE human PC3 cells with BAG6 antibody (green) and anti Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human lung cancer tissue with BAG6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with BAG6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with BAG6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BAG6 antibody.