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Home >> Antibodies >> BAG3 Antibody

BAG3 Antibody (R31850)

  Catalog No Formulation Size Price (USD)  
Image R31850 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) rat brain, 2) rat testis, 3) human MCF7 and 4) human HeLa lysate with BAG3 antibody. Predicted molecular weight ~62 kDa but can be observed at 75~80 kDa.
IHC testing of FFPE human lung cancer tissue with BAG3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt O95817
Localization Cytoplasmic, some nuclear
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This BAG3 antibody is available for research use only.
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Description

BAG3 inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. [UniProt]

Application Notes

Optimal dilution of the BAG3 antibody should be determined by the researcher.

Immunogen

Amino acids 100-561 of human BAG3 were used as the immunogen for the BAG3 antibody.

Storage

After reconstitution, the BAG3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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