- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
BAF47 Antibody rINI1/8850 / SMARCB1 MAb [clone rINI1/8850] (V4861)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V4861-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V4861-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
|
|
V4861SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rINI1/8850 |
| Purity | Protein A/G affinity |
| UniProt | Q12824 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This BAF47 Antibody rINI1/8850 is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
BAF47 Antibody rINI1/8850 recognizes BRG1-associated factor 47 (BAF47), a nuclear protein encoded by the SMARCB1 gene. Also known as INI1, SNF5, and Integrase Interactor 1, this protein is an essential component of mammalian SWI/SNF chromatin-remodeling complexes, also called BAF complexes. These ATP-dependent molecular assemblies regulate chromatin accessibility and influence transcription, cellular differentiation, and tumor suppression.
SMARCB1 participates in chromatin remodeling by supporting the structural integrity and regulatory activity of SWI/SNF complexes. These complexes use energy from ATP hydrolysis to reposition or reorganize nucleosomes, allowing transcription factors and other regulatory proteins to access specific genomic regions. Through these interactions, BAF47 contributes to the control of gene expression programs involved in cell proliferation, lineage commitment, and normal tissue development.
Integrase Interactor 1 is particularly important in cancer biology because SMARCB1 functions as a tumor suppressor. Biallelic inactivation of SMARCB1 is a defining molecular alteration in many malignant rhabdoid tumors, including atypical teratoid/rhabdoid tumors of the central nervous system. Loss of SMARCB1 disrupts normal chromatin regulation and can promote abnormal transcriptional programs associated with malignant transformation.
Immunohistochemical detection of BAF47 is valuable in surgical pathology research because normal cells generally exhibit nuclear INI1 expression, whereas SMARCB1-deficient tumors frequently demonstrate complete loss of tumor cell nuclear staining. Retained nuclear staining in adjacent non-neoplastic cells provides an important internal positive control. This distinction helps researchers investigate SMARCB1-deficient neoplasms and differentiate tumors with similar histological appearances.
Loss of INI1 expression is also associated with epithelioid sarcoma, renal medullary carcinoma, and selected other malignancies. However, the absence of nuclear staining must be interpreted alongside tissue morphology, internal controls, and additional molecular or immunohistochemical findings. Not all tumors within these diagnostic categories share identical SMARCB1 alterations.
BAF47 is therefore an important marker for investigating chromatin-remodeling abnormalities, tumor suppressor pathways, and the molecular classification of SMARCB1-deficient cancers. Its nuclear localization also makes immunohistochemistry useful for examining protein expression in preserved tissue architecture.
A BAF47 Antibody rINI1/8850 from NSJ Bioreagents supports research involving SMARCB1 expression, INI1 nuclear localization, SWI/SNF chromatin remodeling, and the identification of tumors associated with loss of BAF47 protein.
For an antibody with protein microarray specificity validation, see our INI1 Antibody SMARCB1/3984.
Optimal dilution of the BAF47 Antibody rINI1/8850 should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the BAF47 Antibody rINI1/8850.
Aliquot the BAF47 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
SMARCB1 antibody, INI1 antibody, Integrase Interactor 1 antibody, SNF5 antibody, hSNF5 antibody, BAF47 protein antibody, BRG1-Associated Factor 47 antibody, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily B Member 1 antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
