- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
B7-H1 Antibody for WB / PD-L1 (R32579)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R32579 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide |
| UniProt | Q9NZQ7 |
| Localization | Cytoplasmic, membranous |
| Applications | Western Blot : 0.5-1ug/ml |
| Limitations | This B7-H1 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Programmed death-ligand 1 (PD-L1), encoded by the CD274 gene, is a type I transmembrane immune checkpoint protein belonging to the B7 family of immunoregulatory molecules. B7-H1 Antibody for WB recognizes this widely studied immune checkpoint ligand, which is commonly referred to in the literature as PD-L1, CD274, or B7-H1. PD-L1 functions as the primary ligand for the programmed cell death protein 1 (PD-1) receptor expressed on activated T lymphocytes. Engagement of PD-L1 with PD-1 delivers inhibitory signals that suppress T cell proliferation, cytokine production, and cytotoxic responses, thereby helping regulate immune activation and maintain immune tolerance in peripheral tissues.
In biochemical studies, PD-L1 is frequently analyzed using western blot assays because its expression and post-translational modification status provide valuable information about immune signaling pathways. B7-H1 Antibody for WB is particularly suited for protein detection studies focused on CD274 expression levels in cell lysates or tissue extracts. The PD-L1 protein contains extracellular immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail. It undergoes extensive post-translational glycosylation, a modification that influences stability, trafficking, and immune checkpoint activity. As a result, western blot analysis commonly reveals multiple bands representing different glycosylated forms of the PD-L1 protein. Deglycosylation treatments in biochemical experiments frequently reduce these higher molecular weight species to the core PD-L1 protein, helping researchers confirm protein identity and modification status during experimental analysis.
The CD274 gene is located on chromosome 9p24.1 and is transcriptionally regulated by inflammatory signaling pathways. Expression of PD-L1 can be strongly induced by cytokines such as interferon-gamma, which activates transcription factors that increase CD274 expression during immune responses. In many tumor types, this pathway is exploited to increase PD-L1 expression on malignant epithelial cells, enabling tumors to suppress anti-tumor immune responses and evade immune surveillance. Because of this role in immune checkpoint signaling, western blot analysis of PD-L1 expression is commonly used in studies investigating immune regulation, inflammatory signaling, and tumor immunology.
Several well-established literature synonyms exist for this immune checkpoint ligand, including PD-L1, Programmed death-ligand 1, B7-H1, and PDCD1 ligand 1. These alternate names are frequently used across immunology, oncology, and molecular biology research. Western blot detection of CD274 using a rabbit polyclonal antibody provides a useful approach for analyzing PD-L1 protein expression and evaluating immune checkpoint pathway regulation in experimental systems. This rabbit polyclonal B7-H1 antibody is suitable for western blot detection of PD-L1 expression in research applications and is available from NSJ Bioreagents.
Optimal dilution of the B7-H1 antibody for WB should be determined by the researcher.
Amino acids 41-69 (KFPVEKQLDLAALIVYWEMEDKNIIQFVH-human) were used as the immunogen for the B7-H1 antibody.
After reconstitution, the B7-H1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
