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Home >> Antibodies >> B7-H1 Antibody / B7 homolog 1 / PD-L1 / CD274

B7-H1 Antibody / B7 homolog 1 / PD-L1 / CD274 [clone PDL1/8809R] (V5293)

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  Catalog No Formulation Size Price (USD)  
Image V5293-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5293-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5293SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human placental tissue with B7-H1 antibody (clone PDL1/8809R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with B7-H1 antibody (clone PDL1/8809R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free B7-H1 antibody (clone PDL1/8809R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PDL1/8809R
Purity Protein A/G affinity
UniProt Q9NZQ7
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This B7-H1 antibody is available for research use only.
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Description

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Application Notes

Optimal dilution of the B7-H1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 190-290) from the human protein was used as the immunogen for the B7-H1 antibody.

Storage

Aliquot the B7-H1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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