- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
B7-H1 Antibody Recombinant Rabbit MAb PDL1/8809R / B7 homolog 1 / PD-L1 [clone PDL1/8809R] (V5293)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5293-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V5293-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
|
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V5293SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | PDL1/8809R |
| Purity | Protein A/G affinity |
| UniProt | Q9NZQ7 |
| Localization | Cell Surface, Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This B7-H1 antibody is available for research use only. |
|
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CD274 antibody, also known as B7-H1 antibody and Programmed death-ligand 1 antibody, recognizes CD274, an immune checkpoint protein commonly referred to as PD-L1 or B7-H1. B7-H1 Antibody Recombinant Rabbit MAb PDL1/8809R targets this cell surface immunoregulatory molecule encoded by the CD274 gene. CD274, frequently described in the literature as PD-L1, Programmed death-ligand 1, or PDCD1 ligand 1, is a type I transmembrane glycoprotein that regulates immune responses through interaction with the PD-1 receptor on T lymphocytes. Because of this biology, PD-L1 antibody detection is widely used in studies of immune checkpoint signaling, tumor immune evasion, and immune regulation within epithelial and lymphoid tissues.
CD274 is a member of the B7 family of immune regulatory ligands and functions primarily through binding to PD-1, the inhibitory receptor encoded by the PDCD1 gene on activated T cells. Engagement of PD-L1 with PD-1 suppresses T cell activation, proliferation, and cytokine production, thereby limiting immune responses and maintaining peripheral immune tolerance. While this pathway normally protects tissues from excessive immune activation, many tumors exploit the PD-1/PD-L1 checkpoint pathway by upregulating PD-L1 expression on tumor cells. Increased expression of this immune checkpoint ligand suppresses anti-tumor immune responses and contributes to immune escape mechanisms within the tumor microenvironment. For this reason, B7-H1 antibody reagents are widely used in research investigating immune checkpoint biology and tumor immunology.
The CD274 gene is located on chromosome 9p24.1 and encodes a transmembrane glycoprotein containing extracellular immunoglobulin-like domains characteristic of B7 family proteins. Under physiological conditions, PD-L1 expression can be detected on antigen-presenting cells such as macrophages and dendritic cells, as well as on some epithelial and endothelial cells. Expression is strongly induced by inflammatory cytokines, particularly interferon-gamma, which activates signaling pathways that increase PD-L1 transcription during immune responses. In many cancers, constitutive PD-L1 expression is observed in tumor epithelial cells and tumor-associated immune cells, linking CD274 expression to regulation of immune responses within the tumor microenvironment.
Several strong literature synonyms are commonly used for this protein, including PD-L1, Programmed death-ligand 1, B7-H1, and PDCD1 ligand 1. These established names help ensure consistent identification of the CD274 immune checkpoint molecule across immunology, oncology, and pathology research. In tissue-based studies, PD-L1 antibody staining is typically observed as membranous signal in epithelial cells and immune cell populations where the protein functions as a cell surface ligand regulating T cell activity. Clone PDL1/8809R is a recombinant rabbit monoclonal antibody designed to recognize PD-L1 protein expression in relevant experimental systems. This B7-H1 antibody is available from NSJ Bioreagents for investigators studying immune checkpoint signaling, tumor immunology, and immune regulation in epithelial and lymphoid tissues.
Optimal dilution of the B7-H1 antibody recombinant rabbit mAb PDL1/8809R should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 190-290) from the human protein was used as the immunogen for the recombinant B7-H1 antibody.
Aliquot the recombinant B7-H1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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