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Home >> Antibodies >> B2M Antibody / Beta 2 Microglobulin

B2M Antibody / Beta 2 Microglobulin (RQ5644)

  Catalog No Formulation Size Price (USD)  
Image RQ5644 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
Immunofluorescent staining of FFPE rat NRK cells with B2M antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of rat C6 cells with B2M antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B2M antibody.
Western blot testing of rat 1) thymus, 2) lung , 3) spleen, 4) stomach and 5) small intestine lysate with B2M antibody. Predicted molecular weight ~14 kDa.
Availability 1-3 business days
Species Reactivity Rat
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P07151
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This B2M antibody is available for research use only.
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Description

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Application Notes

Optimal dilution of the B2M antibody should be determined by the researcher.

Immunogen

Recombinant rat protein (amino acids I21-M119) was used as the immunogen for the B2M antibody.

Storage

After reconstitution, the B2M antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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