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Home >> Antibodies >> B-RAF Antibody

B-RAF Antibody (F50793)

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  Catalog No Formulation Size Price (USD)  
Image F50793-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50793-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa
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Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa
IHC analysis of FFPE human brain tissue stained with B-RAF antibody
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P15056
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This B-RAF antibody is available for research use only.
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Description

B-RAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Application Notes

Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 379-408 from the human protein was used as the immunogen for this B-RAF antibody.

Storage

Aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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