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Home >> Antibodies >> B-RAF Antibody

B-RAF Antibody (F50792)

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  Catalog No Formulation Size Price (USD)  
Image F50792-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50792-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent image of C2C12 cell stained with B-RAF antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
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Fluorescent image of C2C12 cell stained with B-RAF antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
Western blot analysis of B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa
IHC analysis of FFPE human prostate carcinoma stained with B-RAF antibody
B-RAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Chicken
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P15056
Applications Immunofluorescence : 1:10-1:50
Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This B-RAF antibody is available for research use only.
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Description

BRAF is a human gene, with the protein more formally known as serine/threonine-protein kinase B-Raf. The protein is involved in sending signals inside cells, which are involved in directing cell growth. Drugs that treat cancers driven by BRAF mutations have been developed. Two of these drugs, Vemurafenib and Dabrafenib are approved by FDA for treatment of late-stage melanoma. [Wiki]

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 424-453 from the human protein was used as the immunogen for this B-RAF antibody.

Storage

Store at 4oC for up to one month. For long term, aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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