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Home >> Antibodies >> b-Catenin Antibody

b-Catenin Antibody [clone 9F2] (V3210)

  Catalog No Formulation Size Price (USD)  
Image V3210-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V3210-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V3210SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
Western blot testing of human 1) A431, 2) A549 and 3) MCF7 cell lysate with Beta Catenin antibody (clone 9F2). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
SDS-PAGE Analysis of Purified, BSA-Free b-Catenin Antibody (clone 9F2). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 9F2
Purity Protein G affinity chromatography
UniProt P35222
Localization Cell surface, cytoplasmic, cell junctions
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Limitations This b-Catenin antibody is available for research use only.
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Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

Application Notes

Titration of the b-Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Chicken b-Catenin was used as the immunogen for the b-Catenin antibody.

Storage

Store the b-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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