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Home >> Antibodies >> b-Catenin Antibody

b-Catenin Antibody [clone 6F9] (V3250)

  Catalog No Formulation Size Price (USD)  
Image V3250-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3250-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3250SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC testing of FFPE human tonsil tissue with b-Catenin antibody (clone 6F9). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
Western blot testing of human HeLa cell lysate with b-Catenin antibody (clone 6F9). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
SDS-PAGE Analysis of Purified, BSA-Free b-Catenin Antibody (clone 6F9). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 6F9
Purity Protein G affinity chromatography
UniProt P35222
Localization Cell surface, cytoplasmic, cell junctions
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This b-Catenin antibody is available for research use only.
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Description

Beta Catenin is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. b-Catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. b-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]

Application Notes

Optimal dilution of the b-Catenin antibody to be determined by the researcher.

Immunogen

Chicken beta-Catenin was used as the immunogen for the b-Catenin antibody.

Storage

Store the b-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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