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Home >> Antibodies >> B-ARK1 Antibody / GRK2

B-ARK1 Antibody / GRK2 (RQ5815)

  Catalog No Formulation Size Price (USD)  
Image RQ5815 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
Flow cytometry testing of human U937 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Flow cytometry testing of rat RH35 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with B-ARK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human ThP-1, 2) monkey COS-7, 3) human Raji, 4) rat lung, 5) mouse NIH 3T3 and 6) mouse RAW264.7 lysate with B-ARK1 antibody. Predicted molecular weight ~79 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P25098
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 2-4ug/ml
Limitations This B-ARK1 antibody is available for research use only.
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Description

Beta adrenergic receptor kinase (beta-ARK or BARK) is a serine/threonine intracellular kinase. The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Application Notes

Optimal dilution of the B-ARK1 antibody should be determined by the researcher.

Immunogen

Amino acids KKELRDAYREAQQLVQRV from the human protein were used as the immunogen for the B-ARK1 antibody.

Storage

After reconstitution, the B-ARK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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