• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> b-Actin Antibody / Beta Actin Antibody

b-Actin Antibody / Beta Actin Antibody (R30971)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R30971 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Western blot testing of 1) zebrafish, 2) chicken heart, 3) chicken liver, 4) chicken brain, 5) monkey heart, 6) monkey lung, 7) monkey kidney and 8) monkey liver tissue lysate with b-Actin antibody. Predicted molecular weight ~48 kDa.
›
Western blot testing of 1) zebrafish, 2) chicken heart, 3) chicken liver, 4) chicken brain, 5) monkey heart, 6) monkey lung, 7) monkey kidney and 8) monkey liver tissue lysate with b-Actin antibody. Predicted molecular weight ~48 kDa.
Western blot testing of 1) human HeLa, 2) human K562, 3) human MCF7, 4) human Jukrat, 5) rat brain, 6) rat kidney, 7) mouse brain and 8) mouse kidney tissue lysate with b-Actin antibody. Predicted molecular weight ~48 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey, Zebrafish, Chicken
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P60709
Applications Western blot : 0.5-1ug/ml
Limitations This b-Actin antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC, IF, ELISA
    Reactivity : Human, Mouse
  • Applications : WB, IHC, ELISA
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC, IF, ELISA
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Bovine, Chicken, Hamster, Pig, Rat, Xenopus
  • Applications : WB, IF, IHC, FACS
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat, Bovine, Pig, Primate, Chicken, Xenopus
  • Applications : WB
    Reactivity : Human, Mouse, Rat, Monkey, Rabbit, Chicken, Dog
  • Applications : WB
    Reactivity : Human, Mouse, Rat, Primate, Dog
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Chicken, Mouse, Pig, Primate, Rat, Xenopus
  • Applications : IF, WB, IHC, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine, Pig, Primate, Chicken, Xenopus
  • Applications : WB, IHC, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Chicken, Drosophila, Hamster, Mouse, Pig, Rat, Xenopus
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Mouse, Primate

Description

b-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.

Application Notes

The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the b-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all Actin antibodies

   2.   View all western blot loading control antibodies

   3.   View all b-Actin antibodies

   4.   View all loading control antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.